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HPTLC fingerprinting and screening of antimicrobial activity of

Pongamia pinnata Pierre.

2
1*
1
Rajani Shirsat , Dr.Ajit Kengar and Dr.Aruna Rai
1 Department of Botany, KET’s V.G Vaze College(Autonomous),Mumbai
2 Former HoD. of Botany, Smt. C.H.M. College, Ulhasnagar,Thane

* Email of corresponding author- ajitkengar@gmail.com

ABSTRACT

Plant secondary metabolites are the alternatives for the control of antibiotic-resistant

pathogens which are highly infectious to humans and plants. Concerning these aspects
present work was carried out with phytochemical studies and antibacterial screening of

Pongamia pinnata Pierre. The Preliminary phytochemical screening and HPTLC studies
were performed with methanolic extracts of seeds and callus. The extracts of the seeds and

callus were developed in the mobile phase of toluene: ethyl acetate (90:10) using standard
procedures and scanned under UV at 254 nm, 366 nm, and visible light at 540 nm. Further,

the extracts of seeds and callus were made in diethyl ether and methanol used for in vitro

antibacterial activity. It was performed against multi-drug resistance organisms such as
Klebsiella pneumoniae, Escherichia coli, Bacillus subtilis, and Staphylococcus aureus. The

organic extracts at concentrations of 0.02 mg/ml - 0.1 mg/ml were taken to study the

inhibition properties. The Pongamia pinnata Pierre HPTLC fingerprint analysis from the
extracts showed a wide range of secondary metabolites. Also, it can be considered as a potent

source as antimicrobial agent.
Keywords: Pongamia pinnata, antibacterial activity, callus, phytochemical screening

HPTLC.
INTRODUCTION

Pongamia pinnata Pierre, is one of the most well-known species belongs to the family

Papilionoideae, subfamily of Leguminosae. It is a medium-sized glabrous tree popularly
known as Karanja in Hindi, Indian Beech in English. It is widely distributed throughout

tropical region of Asia Islands, Australia, India, South-Eastern Asia and locally distributed
throughout the State of Maharashtra (India) along the banks of rivers; very common near the

sea-coast in tidal and beach-forests in Konkan and also along Deccan rivers (Prajapati et al.,
2003).

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