Page 27 - e-Book

ANNEXURE- 1

Bold's Basal Medium (modified) (Stein, 1979)

S. No. Stock Stock solution mL/L

1 KH2PO4 8.75 g/500 mL 10 mL

2 CaCl2.2H2O 12.5 g/500 mL 1 mL
3 MgSO4.7H2O 37.5 g/500 mL 1 mL

4 NaNO3 125 g/500 mL 1 mL

5 K2HPO4 37.5 g/500 mL 1 mL
6 NaCl 12.5 g/500 mL 1 mL

Na2EDTA.2H2O 10 g/L
7 1 mL
KOH 6.2 g/L

FeSO4.7H2O 4.98 g/L 1 mL
8
H2SO4 (concentrated) 1 mL/L

9 Trace Metal Solution See below 1 mL
10 H3BO3 5.75 g/500 mL 0.7 mL
11 F/2 Vitamin Solution See below 1 mL

Stir and adjust the pH to 6.8. Autoclave the medium.

Trace metal solution

S. No. Substance g/Litre

1 H3BO3 2.86 g

2 MnCl2.4H2O 1.81 g

3 ZnSO4.7H2O 0.222 g
4 Na2MoO4.2H2O 0.390 g
5 CuSO4.5H2O 0.079 g

6 Co(NO3)2.6H2O 0.0494 g

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