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F/2 Vitamin solution
Final
Primary stock Concentrated concentration
S. No. Substance
(g/L) stock (g/L) in medium
(mg/L)
Vitamin B12 0.001 0.0001 0.001
1
(Cyanocobalamin)
2 Biotin 0.001 0.0001 0.001
3 Thiamine 0.200 0.0200 0.200
To make the working solution added the following amounts of the stock solutions to 100 mL
of distilled water: Vitamin B12 (0.1 mL), Biotin (1 mL) and Thiamine HCl (20 mg).
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