Page 28 - e-Book

F/2 Vitamins

S. No. Stock Solution

1 Vitamin B12 (Cyanocobalamin) 5mg/5mL distilled H2O
2 Biotin 1 mg/10mL distilled H2O

BG-11 Media (Rippika et al. 1979)

S. No. Stock Stock solution mL/Litre
1 NaNO3 150 g/L 10 mL

2 K2HPO4.3H2O 40 g/L 1 mL

3 MgSO4.7H2O 75 g/L 1 mL
4 CaCl2.2H2O 36 g/L 1 mL

Citric Acid 6 g/L 1 mL
5
combined with Ferric Citrate
6 Na2EDTA.2H2O 1 g/L 1 mL

7 Na2CO3 20 g/L 1 mL

8 Trace Metal solution See below 1 mL
9 F/2 vitamins See below 1 mL

Adjusting the pH of the medium to approximately 7.5 will avoid heavy precipitation.

Trace metal stock solution

S. No. Substance g/Litre
1 H3BO3 2.86 g

2 MnCl2.4H2O 1.81 g

3 ZnSO4.7H2O 0.222 g
4 Na2MoO4.2H2O 0.390 g

5 CuSO4.5H2O 0.079 g

6 Co(NO3)2.6H2O 0.0494 g

18