Page 527 - e-Book

Figure 3B.6 HPTLC densitograph of Pongamia pinnata seed oil showing different peaks of

phytoconstituents

II.Antimicrobial activities of Pongamia pinnata seed and Callus
The methanol extract from seed powder of Pongamia pinnata was found to inhibit the growth

of all the pathogens at 0.06mg/ml concentration. At 0.1mg/ml concentration E. coli , K.
pneumoniae, and B. subtilis were found to be inhibited maximum with 18mm, 18mm, and

20mm zone of inhibition respectively. It was observed that S. aureus was more tolerant at
0.1mg/ml concentration of methanolic extract as the zone of inhibition was only 13mm. The

extract of P. pinnata seed powder in diethyl ether does not show any antimicrobial activity

for the selected pathogens at any of the concentrations tested (Table 4A.4). The result
obtained for P. pinnata can be correlated with the report by Kumar et al. (2007) which states

that ethanolic extract of Pongamia pinnata seeds exhibited moderate antimicrobial activity
against acne-inducing bacteria such as Propionibacterium acnes and Staphylococcus

epidermis.
Table 4A.5 depicts the effect of methanol and diethyl ether extracts of Pongamia pinnata

callus. Gram-positive bacteria B. subtilis and Gram-negative bacteria E. coli were found to be

517